Phosphotyrosine Antibody / P-Tyr

Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the p-Tyr antibody should be determined by the researcher.

1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This mAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Phosphotyrosine conjugated to BSA was used as the immunogen for the p-Tyr antibody.

This p-Tyr antibody is available for research use only.

Depends upon the location of phosphorylated target

Protein G affinity chromatography