SOX10 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V3509SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3509SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b kappa
Antibody Clonality: Monoclonal
Antibody Clone: SOX10/1074
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the SOX-10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 4
Protein array validation of the SOX-10 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX-10 antibody (clone SOX10/1074).<P><P>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
IHC testing of FFPE human melanoma with SOX-10 antibody (clone SOX10/1074). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
3 / 4
Western blot testing of human COLO-38 cell lysate using SOX-10 antibody (SOX10/1074). Predicted molecular weight ~50 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free SOX-10 antibody (clone SOX10/1074) as confirmation of integrity and purity.
❮❯

Protein array validation of the SOX-10 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX-10 antibody (clone SOX10/1074).<P><P>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC testing of FFPE human melanoma with SOX-10 antibody (clone SOX10/1074). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human COLO-38 cell lysate using SOX-10 antibody (SOX10/1074). Predicted molecular weight ~50 kDa.
SDS-PAGE analysis of purified, BSA-free SOX-10 antibody (clone SOX10/1074) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the SOX-10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb recognizes a protein of ~50 kDa identified as SOX10. This mAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX10 is moderately to strongly expressed in desmoplastic melanomas. SOX10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX10 expression is also observed in mast cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 115-269 of the human protein were used as the immunogen for the SOX-10 antibody.
Limitation:
This SOX-10 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P56693