Glycophorin A Antibody

NSJ Bioreagents
Product Code: NSJ-V7130SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7130SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SGPA35
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone SGPA35). Expected molecular weight: routinely observed at ~16/38 kDa.~
2 / 2
IHC testing of FFPE human placenta and Glycophorin A antibody (clone SGPA35). Staini

Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone SGPA35). Expected molecular weight: routinely observed at ~16/38 kDa.~
IHC testing of FFPE human placenta and Glycophorin A antibody (clone SGPA35). Staini

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the Glycophorin A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.
Limitation:
This Glycophorin A antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity chromatography
Uniprot #:
P02724