SERBP1 Antibody / PAI-RBP1

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NSJ Bioreagents
Product Code: NSJ-V7609SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7609SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2c kappa
Antibody Clonality: Monoclonal
Antibody Clone: SERBP1/3496
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the SERBP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human kidney with SERBP1 antibody (clone SERBP1/3496). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human urothelial carcinoma with SERBP1 antibody (clone SERBP1/3496). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 4
SDS-PAGE analysis of purified, BSA-free SERBP1 antibody (clone SERBP1/3496) as confirmation of integrity and purity.
4 / 4
Western blot testing of human 1) K562 and 2) PC-3 cell lysate with SERBP1 antibody. Predicted molecular weight ~45 kDa but observed at 45-60 kDa.
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IHC testing of FFPE human kidney with SERBP1 antibody (clone SERBP1/3496). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human urothelial carcinoma with SERBP1 antibody (clone SERBP1/3496). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free SERBP1 antibody (clone SERBP1/3496) as confirmation of integrity and purity.
Western blot testing of human 1) K562 and 2) PC-3 cell lysate with SERBP1 antibody. Predicted molecular weight ~45 kDa but observed at 45-60 kDa.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
PAI-RBP1 (plasminogen activator inhibitor 1 RNA-binding protein), also known as SERBP1 (SERPINE1 mRNA-binding protein 1), CGI-55, CHD3IP (chromodomain helicase DNA binding protein 3 interacting protein), HABP4L or PAI-RBP1, is a membrane-associated protein that localizes to the nucleus, the perinuclear region of the cytoplasm and the plasma membrane. PAI-RBP1 is believed to play a role in the regulation of mRNA stability, as it specifically binds to the CRS (cyclic nucleotide-responsive sequence) motif of the PAI-1 mRNA and acts to stabilize the mRNA and regulate its expression. In addition, PAI-RBP1 interacts with Mi2-alpha and may be involved in chromatin remodeling. PAI-RBP1 also interacts with PGRMC1 and participates in the transduction of Progesterone's antiapoptotic action in ovarian cell types. The gene encoding PAI-RBP1 is overexpressed in ovarian cancer, suggesting a possible role for PAI-RBP1 in tumorigenesis and tumor metastasis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human partial protein corresponding to amino acids 3-139 was used as the immunogen for the SERBP1 antibody.
Limitation:
This SERBP1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
Q8NC51