S100A4 Antibody / FSP1

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NSJ Bioreagents
Product Code: NSJ-V7344SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V7344SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: S100A4/2750R
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody. Predicted molecular weight ~12 kDa.
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IHC testing of FFPE human placenta with recombinant S100A4 antibody. Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant S100A4 antibody as confirmation of integrity and purity.
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Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody. Predicted molecular weight ~12 kDa.
IHC testing of FFPE human placenta with recombinant S100A4 antibody. Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant S100A4 antibody as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the recombinant S100A4 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.
Limitation:
This recombinant S100A4 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein A affinity chromatography
Uniprot #:
P26447