S100A4 Antibody / FSP1

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NSJ Bioreagents
Product Code: NSJ-V3435SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3435SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: S100A4/1482
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the S100A4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.
2 / 5
IHC testing of FFPE human placenta with S100A4 antibody (clone S100A4/1482). Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling.
3 / 5
Flow cytometry testing of human A549 cells with S100A4 antibody (clone S100A4/1482); Red=isotype control, Blue= S100A4 antibody.
4 / 5
SDS-PAGE analysis of purified, BSA-free S100A4 antibody (clone S100A4/1482) as confirmation of integrity and purity.
5 / 5
IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1482, green) and Reddot nuclear stain (red).
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Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.
IHC testing of FFPE human placenta with S100A4 antibody (clone S100A4/1482). Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling.
Flow cytometry testing of human A549 cells with S100A4 antibody (clone S100A4/1482); Red=isotype control, Blue= S100A4 antibody.
SDS-PAGE analysis of purified, BSA-free S100A4 antibody (clone S100A4/1482) as confirmation of integrity and purity.
IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1482, green) and Reddot nuclear stain (red).

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
Description:
S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Human recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.
Limitation:
This S100A4 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P26447