PGP9.5 / UchL1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V3662SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3662SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rUCHL1/775
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

1 / 5
Immunofluorescent staining of permeabilized human T98G cells with recombinant PGP9.5 antibody cocktail (green, clone rUCHL1/775) and Nucspot (red).
2 / 5
IHC staining of FFPE human cerebellum tissue with recombinant PGP9.5 antibody (clone rUCHL1/775). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 5
Western blot testing of human brain lysate with recombinant PGP9.5 antibody (clone rUCHL1/775). Predicted molecular weight ~25 kDa.
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant PGP9.5 antibody (clone rUCHL1/775). These results demonstrate the foremost specificity of the rUCHL1/775 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free recombinant PGP9.5 antibody (clone rUCHL1/775) as confirmation of integrity and purity.
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Immunofluorescent staining of permeabilized human T98G cells with recombinant PGP9.5 antibody cocktail (green, clone rUCHL1/775) and Nucspot (red).
IHC staining of FFPE human cerebellum tissue with recombinant PGP9.5 antibody (clone rUCHL1/775). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human brain lysate with recombinant PGP9.5 antibody (clone rUCHL1/775). Predicted molecular weight ~25 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant PGP9.5 antibody (clone rUCHL1/775). These results demonstrate the foremost specificity of the rUCHL1/775 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free recombinant PGP9.5 antibody (clone rUCHL1/775) as confirmation of integrity and purity.

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant PGP9.5 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant PGP9.5 antibody.
Limitation:
This recombinant PGP9.5 antibody is available for research use only.
Localization:
Cytoplasmic, ER membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P09936