RORC Antibody

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Optimal dilution of the RORC antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This MAb recognizes a protein of 63kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors ROR alpha and ROR gamma are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50% amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered 'orphan receptors,' due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.

Purified

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Recombinant human RORC protein was used as the immunogen for the RORC antibody. The epitope has been found to be with amino acids 1-50.

This RORC antibody is available for research use only.

Nuclear

Protein G affinity chromatography

P51449