Tyrosinase Antibody

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NSJ Bioreagents
Product Code: NSJ-V3658IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3658IHC-7ML7 ml£534.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rOCA1/812
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC staining of FFPE human Melanoma with recombinant Tyrosinase antibody (clone rOCA1/812). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, or 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.~
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IHC staining of FFPE human Melanoma with recombinant Tyrosinase antibody (clone rOCA1/812). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, or 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.~

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the recombinant Tyrosinase antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant protein was used as the immunogen for this recombinant Tyrosinase antibody.
Limitation:
This recombinant Tyrosinase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P14679