CD146 Antibody / MCAM / MUC18

NSJ Bioreagents
Product Code: NSJ-V3638IHC
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3638IHC-7ML7 ml£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rMUC18/1130
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store the recombinant CD146 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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IHC testing of FFPE human tonsil with CD146 antibody (clone rMUC18/1130). Required boiling tissue sections in 10mM Tris with 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
SDS-PAGE analysis of purified, BSA-free reco

IHC testing of FFPE human tonsil with CD146 antibody (clone rMUC18/1130). Required boiling tissue sections in 10mM Tris with 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.~
SDS-PAGE analysis of purified, BSA-free reco

Documents

Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the recombinant CD146 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant CD146 antibody.
Limitation:
This recombinant CD146 antibody is available for research use only.
Localization:
Cell surface, cytoplasm
Purity:
Protein G affinity chromatography
Uniprot #:
P43121