EpCAM Antibody

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NSJ Bioreagents
Product Code: NSJ-V3464SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V3464SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rMOC-31
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Images

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Immunofluorescence testing of human MCF7 cells with recombinant EpCAM antibody (green, clone rMOC-31) and RedDot nuclear stain (red).
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IHC testing of FFPE human colon carcinoma with recombinant EpCAM antibody (clone rMOC-31). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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Flow cytometry testing of PFA-fixed human MCF7 cells with recombinant EpCAM antibody (clone rMOC-31); Red=isotype control, Blue= recombinant EpCAM antibody.
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Immunofluorescence testing of human MCF7 cells with recombinant EpCAM antibody (green, clone rMOC-31) and RedDot nuclear stain (red).
IHC testing of FFPE human colon carcinoma with recombinant EpCAM antibody (clone rMOC-31). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of PFA-fixed human MCF7 cells with recombinant EpCAM antibody (clone rMOC-31); Red=isotype control, Blue= recombinant EpCAM antibody.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for the recombinant EpCAM antibody. Binding epitope of the rMOC-31 antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59.
Limitation:
This recombinant EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P16422