His Tag Chimeric Human Antibody

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NSJ Bioreagents
Product Code: NSJ-R20303
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20303-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RMH01
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Store the recombinant His-Tag Chimeric Human antibody at -20oC.

Images

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Western blot testing of recombinant His-Tag Chimeric Human antibody (0.2ug/ml) with lysate from HEK293 cells transfected (+) or untransfected (-) with a His-Tag Histone H3 DNA construct. An anti-Human IgG secondary mAb was used as detect.
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Sandwich ELISA using recombinant His Tag Chimeric Human antibody at capture, His-Tag Histone H3 (K36M) protein, and anti Histone H3 antibody as detect.
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A titer ELISA using a PCSK9-His protein coated plate, recombinant His-Tag Chimeric Human antibody as the detect and anti-human IgG-HRP as the secondary.
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Western blot testing of recombinant His-Tag Chimeric Human antibody (0.2ug/ml) with lysate from HEK293 cells transfected (+) or untransfected (-) with a His-Tag Histone H3 DNA construct. An anti-Human IgG secondary mAb was used as detect.
Sandwich ELISA using recombinant His Tag Chimeric Human antibody at capture, His-Tag Histone H3 (K36M) protein, and anti Histone H3 antibody as detect.
A titer ELISA using a PCSK9-His protein coated plate, recombinant His-Tag Chimeric Human antibody as the detect and anti-human IgG-HRP as the secondary.

Documents

Further Information

Application Details:
ELISA (Capture): 0. 1 ug/ml-2 ug/ml,ELISA (Detect): 0.01 ug/ml-0.5 ug/ml,Western Blot: 0.1 ug/ml-1 ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant His-Tag Chimeric Human antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody is a Human-Rabbit chimeric antibody made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-His Tag monoclonal antibody (RM146), with the constant domain of Human IgG1. The mAb is recognized only by an anti-Human IgG secondary antibody.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A mixture of a peptides with 6xHis-Tag at the N-terminus and peptides with 6xHis-Tag at the C-terminus was used as the immunogen for the recombinant His-Tag Chimeric Human antibody.
Limitation:
This recombinant His-Tag Chimeric Human antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant