Rabbit IgG Fc Antibody

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NSJ Bioreagents
Product Code: NSJ-R20173BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20173BTN-50UG50ug£513.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: Goat IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RMG02
Regulatory Status: RUO
Target Species: Rabbit
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Rabbit IgG Fc antibody at -20oC.

Images

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ELISA of IgGs from different species shows the recombinant Rabbit IgG Fc antibody reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG.
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Western blot of nonreduced(-) and reduced(+) rabbit IgG (20ng/lane), using 0.2ug/ml of the recombinant Rabbit IgG Fc antibody. This antibody reacts to nonreduced rabbit IgG (~150 kDa).
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ELISA Titration: the plate was coated with different amounts of rabbit IgG. A serial dilution of the recombinant Rabbit IgG Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
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ELISA of IgGs from different species shows the recombinant Rabbit IgG Fc antibody reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG.
Western blot of nonreduced(-) and reduced(+) rabbit IgG (20ng/lane), using 0.2ug/ml of the recombinant Rabbit IgG Fc antibody. This antibody reacts to nonreduced rabbit IgG (~150 kDa).
ELISA Titration: the plate was coated with different amounts of rabbit IgG. A serial dilution of the recombinant Rabbit IgG Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.01ug/ml - 0.5ug/ml,Western Blot (non-reduced): 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Rabbit IgG Fc antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Rabbit IgG Fc antibody reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Rabbit IgG was used as the immunogen for this recombinant Rabbit IgG Fc antibody.
Limitation:
This recombinant Rabbit IgG Fc antibody is available for research use only.
Purity:
Protein G purified from animal origin-free supernatant