Placental Alkaline Phosphatase Antibody / ALPP

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NSJ Bioreagents
Product Code: NSJ-R20336
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20336-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM317
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant PLAP antibody at -20oC.

Images

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Western blot testing of human A431 cell lysate with recombinant PLAP antibody at 1:2000 dilution. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.~
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IHC testing of formalin fixed and paraffin embedded human plac
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Western blot testing of human A431 cell lysate with recombinant PLAP antibody at 1:2000 dilution. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.~
IHC testing of formalin fixed and paraffin embedded human plac

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:500-1:1000,Western Blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant PLAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.

PLAP is a tumor marker, especially in seminoma and ovarian cancer. PLAP is reliable only in non-smokers, as smoking interferes with measurement of PLAP, since serum concentrations of PLAP are increased up to 10-fold in smokers and its measurement is therefore of little value in this group. [Wiki]
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the N-terminus of human placental alkaline phosphatase was used as the immunogen for the recombinant PLAP antibody.
Limitation:
This recombinant PLAP antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P05187