HA Tag Antibody

NSJ Bioreagents
Product Code: NSJ-R20304
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20304-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM305
Regulatory Status: RUO
Applications:
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant HA Tag antibody at -20oC.

Images

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Western blot testing of recombinant HA Tag antibody (0.1ug/ml) with lysate from HEK293 cells transfected (+) or untransfected (-) with an HA-tag KRas DNA construct.~
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IHC staining of formalin fixed and paraffin embedded human LNCaP cell

Western blot testing of recombinant HA Tag antibody (0.1ug/ml) with lysate from HEK293 cells transfected (+) or untransfected (-) with an HA-tag KRas DNA construct.~
IHC staining of formalin fixed and paraffin embedded human LNCaP cell

Documents

Further Information

Application Details:
Western Blot: 0.1 ug/ml-1 ug/ml,Immunoprecipitation: 0.5 ug/ml-2 ug/ml,Immunocytochemistry: 0.1 ug/ml-1 ug/ml,Flow Cytometry: 0.1-1 ug/million cells,Immunohistochemistry: 0.1 ug/ml-0.5 ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant HA Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106) was used as the immunogen for the recombinant HA Tag antibody.
Limitation:
This recombinant HA Tag antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant