S100B Antibody (C-Terminal)

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NSJ Bioreagents
Product Code: NSJ-R20326
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20326-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM304
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant S100B antibody at -20oC.

Images

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Western blot testing of human brain tissue lysate with recombinant S100B antibody at 1:100 dilution. Predicted molecular weight ~11 kDa.
2 / 3
IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant S100B antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant S100B antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human brain tissue lysate with recombinant S100B antibody at 1:100 dilution. Predicted molecular weight ~11 kDa.
IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant S100B antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant S100B antibody at 1:1000 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:500-1:1000,Western Blot: 1:100-1:200
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant S100B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human S100-B (S-100 protein beta chain).
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human S100B was used as the immunogen for the recombinant S100B antibody.
Limitation:
This recombinant S100B antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P04271