p27 Antibody / p27Kip1 (C-Terminal)

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NSJ Bioreagents
Product Code: NSJ-R20324
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20324-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM302
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant p27 antibody at -20oC.

Images

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IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant p27 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.~
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Western blot tes
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IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant p27 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.~
Western blot tes

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100-1:200,Western Blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant p27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human p27Kip1 (Cyclin-dependent kinase inhibitor 1B). It may also react to mouse p27Kip1, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human p27Kip1 was used as the immunogen for the recombinant p27 antibody.
Limitation:
This recombinant p27 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P46527