EIF2A Antibody (pS51)

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NSJ Bioreagents
Product Code: NSJ-R20320
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20320-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM298
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant phospho-EIF2A antibody at -20oC.

Images

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Western blot testing of lysate from human HeLa cells, untreated (-) or treated (+) with Calyculin A (CalA), with recombinant phospho-EIF2A antibody at 1:200 dilution. Predicted molecular weight ~36 kDa.
2 / 3
IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant phospho-EIF2A antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
IHC testing of formalin fixed and paraffin embedded human thyroid cancer tissue with recombinant phospho-EIF2A antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of lysate from human HeLa cells, untreated (-) or treated (+) with Calyculin A (CalA), with recombinant phospho-EIF2A antibody at 1:200 dilution. Predicted molecular weight ~36 kDa.
IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant phospho-EIF2A antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of formalin fixed and paraffin embedded human thyroid cancer tissue with recombinant phospho-EIF2A antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100-1:200,Western Blot: 1:100-1:200
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EIF2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human eIF-2a (Eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. There is no cross-reactivity to eIF-2a without phosphorylation at Ser51.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding the the amino acids surrounding phosphorylated serine 51 was used as the immunogen for the recombinant phospho-EIF2A antibody.
Limitation:
This recombinant phospho-EIF2A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P05198