Aurora B Antibody / AURKB

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NSJ Bioreagents
Product Code: NSJ-R20295
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20295-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM278
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Aurora B antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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IHC testing of FFPE human tonsil tissue with recombinant Aurora B antibody at 1:1000.~
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Western blot of HeLa cell lysate, untreated (-) or treated (+) with Nocodazole (NOC), using recombinant Aurora B antibody at 1:1000. Predicted molec
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IHC testing of FFPE human tonsil tissue with recombinant Aurora B antibody at 1:1000.~
Western blot of HeLa cell lysate, untreated (-) or treated (+) with Nocodazole (NOC), using recombinant Aurora B antibody at 1:1000. Predicted molec

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:200-1:500 (1),Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Aurora B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Aurora B antibody reacts to human Aurora kinase B.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the N-terminus of human Aurora kinase B was used as the immunogen for this recombinant Aurora B antibody.
Limitation:
This recombinant Aurora B antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q96GD4