SMAD4 Antibody

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NSJ Bioreagents
Product Code: NSJ-R20294
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20294-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM277
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant SMAD4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of human Jurkat and IMR32 cell lysate using recombinant SMAD4 antibody at 1:500. Predicted molecular weight ~60 kDa.~
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IHC testing of FFPE human breast cancer tissue with recombinant SMAD4 antibody at 1:2000.
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Western blot of human Jurkat and IMR32 cell lysate using recombinant SMAD4 antibody at 1:500. Predicted molecular weight ~60 kDa.~
IHC testing of FFPE human breast cancer tissue with recombinant SMAD4 antibody at 1:2000.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:1000-1:2000 (1),Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant SMAD4 antibody reacts to human SMAD4 and may also react to mouse or rat Smad4, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human SMAD4 was used as the immunogen for this recombinant SMAD4 antibody.
Limitation:
This recombinant SMAD4 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q13485