Phospho-EGF Receptor / EGFR Antibody (pY1173)
Code | Size | Price |
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NSJ-R20286-0.1ML | 100ul | £524.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM269
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store the recombinant phospho-EGFR antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Further Information
Application Details:
Immunohistochemistry (FFPE): 1:500-1:1000 (1),Western blot: 1:1000-1:5000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Description:
This recombinant phospho-EGFR antibody reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to human phospho- EGFR (Tyr1173) was used as the immunogen for this recombinant phospho-EGFR antibody.
Limitation:
This recombinant phospho-EGFR antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P00533