Alpha Actin Antibody / ACTC1 (cardiac muscle)

NSJ Bioreagents
Product Code: NSJ-R20278
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20278-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM257
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant ACTC1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot testing of mouse heart lysate with recombinant ACTC1 antibody at 1:1000. Predicted molecular weight ~42 kDa.~
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IHC testing of FFPE human heart tissue with recombinant ACTC1 antibody at 1:1000.

Western blot testing of mouse heart lysate with recombinant ACTC1 antibody at 1:1000. Predicted molecular weight ~42 kDa.~
IHC testing of FFPE human heart tissue with recombinant ACTC1 antibody at 1:1000.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:1000-1:2000 (1),Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant ACTC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
The recombinant ACTC1 antibody reacts to human and mouse alpha cardiac actin (Actin, alpha cardiac muscle 1). It may also react to bovine or rat alpha-cardiac actin, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the N-terminus of human alpha-cardiac Actin was used as the immunogen for this recombinant ACTC1 antibody.
Limitation:
This recombinant ACTC1 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P68032