Alpha Actin Antibody / ACTA2 (smooth muscle)

NSJ Bioreagents
Product Code: NSJ-R20272
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20272-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM253
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant ACTA2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot testing of mouse heart lysate with recombinant ACTA2 antibody at 1:1000. Predicted molecular weight ~42 kDa.
2 / 3
IHC testing of FFPE human heart tissue with recombinant ACTA2 antibody at 1:2500.
3 / 3
IHC testing of FFPE human kidney tissue with recombinant ACTA2 antibody at 1:2500.

Western blot testing of mouse heart lysate with recombinant ACTA2 antibody at 1:1000. Predicted molecular weight ~42 kDa.
IHC testing of FFPE human heart tissue with recombinant ACTA2 antibody at 1:2500.
IHC testing of FFPE human kidney tissue with recombinant ACTA2 antibody at 1:2500.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:1000-1:2500 (1),Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Alpha Smooth Muscle Actin / ACTA2 antibody reacts to human and mouse alpha smooth muscle Actin (alpha-Actin-2). It may also react to bovine or rat alpha smooth muscle Actin, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the N-terminus of human alpha smooth muscle Actin was used as the immunogen for this recombinant ACTA2 antibody.
Limitation:
This recombinant ACTA2 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P62736