AKT Antibody (pSer473)
| Code | Size | Price |
|---|
| NSJ-R20271-0.1ML | 100ul | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM251
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store the recombinant phospho-AKT antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Documents
Further Information
Application Details:
Immunohistochemistry (FFPE): 1:200-1:500 (1),Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Description:
This recombinant phospho-AKT antibody reacts to AKT1/2/3 only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. It may also react to bovine, mouse or rat phospho-AKT (Ser473), as predicted by immunogen homology
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to human phospho-Akt (Ser473) was used as the immunogen for this recombinant phospho-AKT antibody.
Limitation:
This recombinant phospho-AKT antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P31749, P31751, Q9Y243