HIF1A Antibody / HIF-1 alpha

Product Code: NSJ-R20263

Code	Size	Price

NSJ-R20263-0.1ML

100ul

£524.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Recombinant Antibody

Antibody Clone: RM242

Regulatory Status: RUO

Target Species: Human

Applications:

Immunohistochemistry (IHC)
Western Blot (WB)

Storage:

Store the recombinant HIF1A antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 2

Western blot test of Jurkat cell lysate, untreated or treated with Cobalt Chloride (CoCl2), using recombinant HIF1A antibody at 1:1000. Routinely observed molecular weight: 100~120 kDa.~

2 / 2

IHC testing of FFPE human breast cancer tissue w

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Documents

Datasheet

Further Information

Application Details:

Immunohistochemistry (FFPE): 1:500-1:1000 (1),Western blot: 1:1000-1:2000

Application Note:

The stated application concentrations are suggested starting points. Titration of the recombinant HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:

This recombinant HIF1A antibody reacts to human Hypoxia-inducible factor 1-alpha.

Format:

Purified

Formulation:

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Immunogen:

A peptide corresponding to Hypoxia-inducible factor 1-alpha was used as the immunogen for this recombinant HIF1A antibody.

Limitation:

This recombinant HIF1A antibody is available for research use only.

Localization:

Nuclear, possible cytoplasmic

Purity:

Protein A purified from animal origin-free supernatant

Uniprot #:

Q16665