5-Hydroxymethylcytosine Antibody (5hmC)
Code | Size | Price |
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NSJ-R20246-50UG | 50ug | £615.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM236
Regulatory Status: RUO
Target Species: Human
Applications:
- Dot Blot
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- hmEdIP
Storage:
Store the recombinant 5hmC antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Further Information
Application Details:
Dot blot: 0.2-1ug/ml,ELISA: 0.1-1ug/ml,Immunohistochemistry: 0.1-1ug/ml (1),Immunocytochemistry: 0.5-2ug/ml,hMeDIP: 0.2ug/ml- 2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Description:
This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.
Limitation:
This recombinant 5hmC antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant