RSK1 Antibody (pThr359/pSer363)

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NSJ Bioreagents
Product Code: NSJ-R20260
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20260-0.1ML100ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM233
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant phospho-RSK1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of A431 cells treated (+) or untreated (-) with EGF, using recombinant phospho-RSK1 antibody at 1:1000. Predicted molecular weight: 83-90 kDa.~
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IHC testing of FFPE human cerebral cortex tissue with recombinant phospho-RSK1
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Western blot of A431 cells treated (+) or untreated (-) with EGF, using recombinant phospho-RSK1 antibody at 1:1000. Predicted molecular weight: 83-90 kDa.~
IHC testing of FFPE human cerebral cortex tissue with recombinant phospho-RSK1

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:200-1:500 (1),Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-RSK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant phospho-RSK1 antibody reacts to human RSK1 only when phosphorylated at Thr359/Ser363.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to human phospho-Rsk1 (Thr359/Ser363) was used as the immunogen for this recombinant phospho-RSK1 antibody.
Limitation:
This recombinant phospho-RSK1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q15418