HER2 Antibody / ErbB2

NSJ Bioreagents
Product Code: NSJ-R20258
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20258-0.1ML0.1ml£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM228
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant HER2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of MCF7, PC3, Jurkat, and A375 cell lysate using recombinant HER2 antibody. Only the breast cancer line MCF7 showed a response.~
2 / 2
IHC testing of FFPE human breast cancer tissue with recombinant HER2 antibody. Expected molec

Western blot of MCF7, PC3, Jurkat, and A375 cell lysate using recombinant HER2 antibody. Only the breast cancer line MCF7 showed a response.~
IHC testing of FFPE human breast cancer tissue with recombinant HER2 antibody. Expected molec

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100 - 1:400 (1),Western blot: 1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant HER2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant HER2 antibody reacts to Human c-erbB-2/HER-2. It may also react to mouse or rat c-erbB-2/HER-2, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human c-erbB2/HER2 was used as the immunogen for this recombinant HER2 antibody.
Limitation:
This recombinant HER2 antibody is available for research use only.
Localization:
Cell surface, cytoplasm, nucleus
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P04626