Mouse IgG2c Antibody

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NSJ Bioreagents
Product Code: NSJ-R20171BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20171BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM223
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG2c antibody at -20oC.

Images

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ELISA of IgG from BalB/C and C57BL6 shows the recombinant Mouse IgG2c antibody reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a.
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Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of the recombinant Mouse IgG2c antibody. This mAb reacts to nonreduced IgG2c.
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ELISA Titration: the plate was coated with different amounts of mouse IgG2c. A serial dilution of the recombinant Mouse IgG2c antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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ELISA of IgG from BalB/C and C57BL6 shows the recombinant Mouse IgG2c antibody reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a.
Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of the recombinant Mouse IgG2c antibody. This mAb reacts to nonreduced IgG2c.
ELISA Titration: the plate was coated with different amounts of mouse IgG2c. A serial dilution of the recombinant Mouse IgG2c antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.01ug/ml - 0.2ug/ml,Western Blot (non-reduced only): 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2c antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG2c antibody reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG2c was used as the immunogen for this recombinant Mouse IgG2c antibody.
Limitation:
This recombinant Mouse IgG2c antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant