Mouse IgG2c Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R20171
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20171-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM223
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG2c antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 3
Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of the recombinant Mouse IgG2c antibody. This mAb reacts to nonreduced IgG2c.
2 / 3
ELISA of IgG from BalB/C and C57BL6 shows the recombinant Mouse IgG2c antibody reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a.
3 / 3
ELISA Titration: the plate was coated with different amounts of mouse IgG2c. A serial dilution of the recombinant Mouse IgG2c antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
❮❯

Western blot of nonreduced(-) and reduced(+) mouse IgG2c, using 0.5ug/mL of the recombinant Mouse IgG2c antibody. This mAb reacts to nonreduced IgG2c.
ELISA of IgG from BalB/C and C57BL6 shows the recombinant Mouse IgG2c antibody reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a.
ELISA Titration: the plate was coated with different amounts of mouse IgG2c. A serial dilution of the recombinant Mouse IgG2c antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.01ug/ml - 0.2ug/ml,Western Blot (non-reduced only): 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2c antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG2c antibody reacts to the Fc region of mIgG2c. No cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG2c was used as the immunogen for this recombinant Mouse IgG2c antibody.
Limitation:
This recombinant Mouse IgG2c antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant