IgA Heavy Chain Antibody

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NSJ Bioreagents
Product Code: NSJ-R20169BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20169BTN-50UG50ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM220
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgA antibody at -20oC.

Images

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Western blot of nonreduced(-) and reduced(+) mouse IgA, using 0.5ug/mL of recombinant Mouse IgA antibody. This antibody reacts to nonreduced IgA.
2 / 3
ELISA of mouse immunoglobulins shows the recombinant Mouse IgA antibody reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.
3 / 3
ELISA Titration: the plate was coated with different amount of mouse IgA. A serial dilution of recombinant Mouse IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgA, using 0.5ug/mL of recombinant Mouse IgA antibody. This antibody reacts to nonreduced IgA.
ELISA of mouse immunoglobulins shows the recombinant Mouse IgA antibody reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.
ELISA Titration: the plate was coated with different amount of mouse IgA. A serial dilution of recombinant Mouse IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.005ug/ml - 0.2ug/ml,Western Blot (non-reduced only): 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgA antibody specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgA was used as the immunogen for this recombinant Mouse IgA antibody.
Limitation:
This recombinant Mouse IgA antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01878