Mouse IgG2a Antibody

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NSJ Bioreagents
Product Code: NSJ-R20166BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20166BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM219
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Mouse IgG2a antibody at -20oC.

Images

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Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5ug/ml of recombinant Mouse IgG2a antibody. This mAb reacts to nonreduced IgG2a (~150 kDa).
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2a antibody reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
3 / 3
ELISA titer: the plate was coated with different amounts of mouse IgG2a. A serial dilution of recombinant Mouse IgG2a antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5ug/ml of recombinant Mouse IgG2a antibody. This mAb reacts to nonreduced IgG2a (~150 kDa).
ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2a antibody reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
ELISA titer: the plate was coated with different amounts of mouse IgG2a. A serial dilution of recombinant Mouse IgG2a antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.005ug/ml - 0.2ug/ml,Western Blot (non-reduced only): 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2a antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Mouse IgG2a antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mouse IgG2a was used as the immunogen for this recombinant Mouse IgG2a antibody.
Limitation:
This recombinant Mouse IgG2a antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01863