Human IgG4 Fc Antibody

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NSJ Bioreagents
Product Code: NSJ-R20191BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20191BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM217
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Store the recombinant Human IgG4 Fc antibody at -20oC.

Images

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Sandwich ELISA with human IgG4 using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
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Sandwich ELISA with human plasma using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
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Recombinant Human IgG4 Fc antibody specifically reacts to hIgG4. No cross reactivity with human IgG1, IgG2, IgG3.
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ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA with human IgG4 using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human plasma using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Recombinant Human IgG4 Fc antibody specifically reacts to hIgG4. No cross reactivity with human IgG1, IgG2, IgG3.
ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 Fc antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgG4 Fc antibody reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Peptide corresponding to the Fc region of human IgG4 was used as the immunogen for this recombinant Human IgG4 Fc antibody.
Limitation:
This recombinant Human IgG4 Fc antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01861