Human IgG4 Fc Antibody

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NSJ Bioreagents
Product Code: NSJ-R20191
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20191-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM217
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Store the recombinant Human IgG4 Fc antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Sandwich ELISA with human plasma using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
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Sandwich ELISA with human IgG4 using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
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Recombinant Human IgG4 Fc antibody specifically reacts to hIgG4. No cross reactivity with IgG1, IgG2, IgG3.
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ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA with human plasma using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human IgG4 using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Recombinant Human IgG4 Fc antibody specifically reacts to hIgG4. No cross reactivity with IgG1, IgG2, IgG3.
ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 Fc antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 Fc antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgG4 Fc antibody reacts to the Fc region of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Peptide corresponding to the Fc region of hIgG4 was used as the immunogen for this recombinant Human IgG4 Fc antibody.
Limitation:
This recombinant Human IgG4 Fc antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01861