His Tag Antibody

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NSJ Bioreagents
Product Code: NSJ-R20255BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20255BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM146
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant His Tag antibody at -20oC.

Images

1 / 3
A titer ELISA performed on purified His-tagged PCSK9 recombinant protein. A serial dilution of biotin labelled His Tag antibody was used as the primary antibody, followed by alkaline phosphatase conjugated Streptavidin.
2 / 3
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 protein, using biotin labelled His Tag antibody at 0.2 ug/ml, followed by a HRP conjugated Streptavidin.
3 / 3
ICC staining of 293T cells expressing His-Tag nuclear protein X (left) and negative control HepG2 cells (right) using the recombinant His Tag antibody.
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A titer ELISA performed on purified His-tagged PCSK9 recombinant protein. A serial dilution of biotin labelled His Tag antibody was used as the primary antibody, followed by alkaline phosphatase conjugated Streptavidin.
Western blot of 293T cells transfected (+) or untransfected (-) with a DNA construct encoding His-Tag Histone H3 protein, using biotin labelled His Tag antibody at 0.2 ug/ml, followed by a HRP conjugated Streptavidin.
ICC staining of 293T cells expressing His-Tag nuclear protein X (left) and negative control HepG2 cells (right) using the recombinant His Tag antibody.

Documents

Further Information

Application Details:
Western Blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-2ug/ml,Flow Cytometry: 0.5-2ug/ml,Immunohistochemistry: 0.1-1ug/ml (1),ELISA: 0.01ug/ml-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant His Tag antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this recombinant His Tag antibody.
Limitation:
This recombinant His Tag antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant