His Tag Antibody

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NSJ Bioreagents
Product Code: NSJ-R20255
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20255-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM146
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant His Tag antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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IP and detect of 293T cells expressing a His-Tag fusion protein using recombinant His Tag antibody. (1) Whole lysate control, (2) IP by rabbit IgG control; (3) IP by RM146 mAb.
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Western blot of 293T cells (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y or (4) E. coli lysate with His-Tag Protein Y. The recombinant His Tag antibody was used at 0.2 ug/ml.
3 / 3
ICC staining of 293T cells expressing His-Tag nuclear protein X (left) and negative control HepG2 cells (right) using the recombinant His Tag antibody.
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IP and detect of 293T cells expressing a His-Tag fusion protein using recombinant His Tag antibody. (1) Whole lysate control, (2) IP by rabbit IgG control; (3) IP by RM146 mAb.
Western blot of 293T cells (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y or (4) E. coli lysate with His-Tag Protein Y. The recombinant His Tag antibody was used at 0.2 ug/ml.
ICC staining of 293T cells expressing His-Tag nuclear protein X (left) and negative control HepG2 cells (right) using the recombinant His Tag antibody.

Documents

Further Information

Application Details:
Western blot: 0.1-1ug/ml,Immunoprecipitation: 0.5-2ug/mg,Immunocytochemistry: 0.5-2ug/ml,Flow cytometry: 0.5-2ug/ml,Immunohistochemistry: 0.1-1ug/ml (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant His Tag antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this recombinant His Tag antibody.
Limitation:
This recombinant His Tag antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant