IgA Heavy Chain Antibody

NSJ Bioreagents
Product Code: NSJ-R20182BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20182BTN-50UG50ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM128
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgA antibody at -20oC.

Images

1 / 4
Sandwich ELISA of human IgA using the recombinant Human IgA antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
2 / 4
Sandwich ELISA of human plasma using the Recombinant Human IgA Antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
3 / 4
ELISA of human immunoglobulins shows the recombinant Human IgA antibody reacts to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE.
4 / 4
ELISA Titration: the plate was coated with different amounts of human IgA. A serial dilution of the recombinant Human IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Sandwich ELISA of human IgA using the recombinant Human IgA antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human plasma using the Recombinant Human IgA Antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody</a>, clone RM129, as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows the recombinant Human IgA antibody reacts to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA. A serial dilution of the recombinant Human IgA antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgA antibody reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgA was used as the immunogen for this recombinant Human IgA antibody.
Limitation:
This recombinant Human IgA antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01876, P01877