Kappa Light Chain Antibody

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NSJ Bioreagents
Product Code: NSJ-R20178
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20178-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM126
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human Kappa Light Chain antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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IHC testing of FFPE human tonsil tissue with recombinant Human Kappa Light Chain antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
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ELISA of human immunoglobulins shows the recombinant Human Kappa Light Chain antibody reacts to the kappa light chain of hIgs. No cross reactivity with the lambda light chain, mouse/rat/goat IgG.
3 / 3
ELISA Titration: the plate was coated with different amounts of human IgG4 kappa;. A serial dilution of the recombinant Human Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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IHC testing of FFPE human tonsil tissue with recombinant Human Kappa Light Chain antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows the recombinant Human Kappa Light Chain antibody reacts to the kappa light chain of hIgs. No cross reactivity with the lambda light chain, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG4 kappa;. A serial dilution of the recombinant Human Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 0.05-0.2ug/ml,Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human Kappa Light Chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human Kappa Light Chain antibody reacts to the kappa light chain of human immunoglobulins. No cross reactivity with lambda or mouse/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgG was used as the immunogen for this recombinant Human Kappa Light Chain antibody.
Limitation:
This recombinant Human Kappa Light Chain antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01834