Human IgA2 Antibody

NSJ Bioreagents
Product Code: NSJ-R20184
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20184-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM125
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgA2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 5
Sandwich ELISA of human plasma using recombinant Human IgA2 antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
2 / 5
Sandwich ELISA of human IgA2 using recombinant Human IgA2 antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
3 / 5
IHC testing of FFPE human tonsil with recombinant Human IgA2 antibody (clone RM125). A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
4 / 5
ELISA of human immunoglobulins shows recombinant Human IgA2 antibody reacts only to IgA2. No cross reactivity with IgA1, IgG, IgM, IgD, or IgE.
5 / 5
ELISA Titration: the plate was coated with different amounts of human IgA2. A serial dilution of recombinant Human IgA2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Sandwich ELISA of human plasma using recombinant Human IgA2 antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human IgA2 using recombinant Human IgA2 antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
IHC testing of FFPE human tonsil with recombinant Human IgA2 antibody (clone RM125). A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows recombinant Human IgA2 antibody reacts only to IgA2. No cross reactivity with IgA1, IgG, IgM, IgD, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgA2. A serial dilution of recombinant Human IgA2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.1-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgA2 antibody reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgA2 was used as the immunogen for this recombinant Human IgA2 antibody.
Limitation:
This recombinant Human IgA2 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01877