IgD Antibody

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NSJ Bioreagents
Product Code: NSJ-R20185BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20185BTN-50UG50ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM123
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgD antibody at -20oC.

Images

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Sandwich ELISA of human plasma using recombinant Human IgD antibody as the capture (100ng/well) and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA of hIgs shows recombinant Human IgD antibody reacts to IgD from human plasma and IgD, kappa; from human myeloma. No cross reactivity with IgG, IgM, IgA, or IgE.
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ELISA Titration: the plate was coated with different amounts of human IgD (from plasma). A serial dilution of recombinant Human IgD antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA of human plasma using recombinant Human IgD antibody as the capture (100ng/well) and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of hIgs shows recombinant Human IgD antibody reacts to IgD from human plasma and IgD, kappa; from human myeloma. No cross reactivity with IgG, IgM, IgA, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgD (from plasma). A serial dilution of recombinant Human IgD antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 25ng/well - 200ng/well (Capture); 0.01 - 0.1ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgD antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgD antibody reacts to hIgD. No cross reactivity with IgG, IgM, IgA, or IgE.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgD was used as the immunogen for this recombinant Human IgD antibody.
Limitation:
This recombinant Human IgD antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01880