Human IgE Antibody

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NSJ Bioreagents
Product Code: NSJ-R20186BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20186BTN-50UG50ug£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM122
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgE antibody at -20oC.

Images

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ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgE lambda; from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.
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Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
3 / 3
ELISA Titration: the plate was coated with different amounts of hIgE. A serial dilution of recombinant Human IgE antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgE lambda; from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.
Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA Titration: the plate was coated with different amounts of hIgE. A serial dilution of recombinant Human IgE antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 10ng/well - 100ng/well (Capture); 0.01 - 0.1ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgE antibody reacts to human IgE. No cross reactivity with human IgG, IgM, IgD, or IgA.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgE was used as the immunogen for this recombinant Human IgE antibody.
Limitation:
This recombinant Human IgE antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01854