Human IgE Antibody

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NSJ Bioreagents
Product Code: NSJ-R20186
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20186-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM122
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgE antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
2 / 3
ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgE lambda; from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.
3 / 3
ELISA Titration: the plate was coated with different amounts of hIgE. A serial dilution of recombinant Human IgE antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgE lambda; from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.
ELISA Titration: the plate was coated with different amounts of hIgE. A serial dilution of recombinant Human IgE antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 10ng/well - 100ng/well (Capture); 0.01 - 0.1ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgE antibody reacts to hIgE. No cross reactivity with IgG, IgM, IgD, or IgA.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgE was used as the immunogen for this recombinant Human IgE antibody.
Limitation:
This recombinant Human IgE antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01854