IgM Heavy Chain Antibody

NSJ Bioreagents
Product Code: NSJ-R20181BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20181BTN-50UG50ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM121
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgM antibody at -20oC.

Images

1 / 4
Sandwich ELISA of human IgM using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
2 / 4
Sandwich ELISA of human plasma using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
3 / 4
ELISA of human immunoglobulins shows the recombinant Human IgM antibody reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.
4 / 4
ELISA Titration: the plate was coated with different amounts of human IgM. A serial dilution of the recombinant Human IgM antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Sandwich ELISA of human IgM using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA of human plasma using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows the recombinant Human IgM antibody reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.
ELISA Titration: the plate was coated with different amounts of human IgM. A serial dilution of the recombinant Human IgM antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.5-2ug/ml (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Human IgM antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgM was used as the immunogen for this recombinant Human IgM antibody.
Limitation:
This recombinant Human IgM antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01871