Human IgG4 Antibody / Hinge region

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NSJ Bioreagents
Product Code: NSJ-R20190
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20190-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM120
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store the recombinant Human IgG4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Sandwich ELISA with human plasma using recombinant Human IgG4 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa; + lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin
2 / 5
Sandwich ELISA with human IgG4 using recombinant Human IgG4 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
3 / 5
Western blot of human, mouse, rat, and goat IgG shows the recombinant Human IgG4 antibody reacts to hIgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of hIgG, or mouse/rat/goat IgG.
4 / 5
IHC testing of FFPE human lymphoid tissue with recombinant Human IgG4 antibody.
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ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA with human plasma using recombinant Human IgG4 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa; + lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin
Sandwich ELISA with human IgG4 using recombinant Human IgG4 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Western blot of human, mouse, rat, and goat IgG shows the recombinant Human IgG4 antibody reacts to hIgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of hIgG, or mouse/rat/goat IgG.
IHC testing of FFPE human lymphoid tissue with recombinant Human IgG4 antibody.
ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 1-10ug/ml,Immunohistochemistry: 1-10ug/ml (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.
Limitation:
This recombinant Human IgG4 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01861