Human IgG3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R20189BTN
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20189BTN-50UG50ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM119
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgG3 antibody at -20oC.

Images

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Sandwich ELISA with human IgG3 using recombinant Human IgG3 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
2 / 5
Sandwich ELISA with human plasma using recombinant Human IgG3 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
3 / 5
IHC testing of FFPE human lymphoid tissue with recombinant Human IgG3 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
4 / 5
ELISA of human immunoglobulins shows recombinant Human IgG3 antibody reacts only to hIgG3. No cross reactivity with IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
5 / 5
ELISA Titration: the plate was coated with different amounts of hIgG3. A serial dilution of recombinant Human IgG3 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA with human IgG3 using recombinant Human IgG3 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human plasma using recombinant Human IgG3 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
IHC testing of FFPE human lymphoid tissue with recombinant Human IgG3 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows recombinant Human IgG3 antibody reacts only to hIgG3. No cross reactivity with IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of hIgG3. A serial dilution of recombinant Human IgG3 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.5-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgG3 antibody reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgG3 was used as the immunogen for this recombinant Human IgG3 antibody.
Limitation:
This recombinant Human IgG3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01860