Human IgG2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R20188
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20188-100UG100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM118
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgG2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Sandwich ELISA with human plasma using recombinant Human IgG2 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
2 / 4
Sandwich ELISA with human IgG2 using recombinant Human IgG2 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
3 / 4
ELISA of human immunoglobulins shows recombinant Human IgG2 antibody only reacted to hIgG2. No cross reactivity with IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
4 / 4
ELISA Titration: the plate was coated with different amounts of human IgG2. A serial dilution of recombinant Human IgG2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Sandwich ELISA with human plasma using recombinant Human IgG2 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human IgG2 using recombinant Human IgG2 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows recombinant Human IgG2 antibody only reacted to hIgG2. No cross reactivity with IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG2. A serial dilution of recombinant Human IgG2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Human IgG2 antibody reacts to the heavy chain of human IgG2. No cross reactivity with IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgG2 was used as the immunogen for this recombinant Human IgG2 antibody.
Limitation:
This recombinant Human IgG2 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01859