Human IgG1 Antibody
Code | Size | Price |
---|
NSJ-R20187-100UG | 100ug | £524.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM117
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Fluorescence-activated cell sorting (FACS)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Further Information
Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.5-2ug/ml (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Description:
This recombinant Human IgG1 antibody reacts to the heavy chain of hIgG1. No cross reactivity with IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Peptide corresponding to the hinge region of human IgG1 was used as the immunogen for this recombinant Human IgG1 antibody.
Limitation:
This recombinant Human IgG1 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01857