IgG Heavy Chain Antibody
Code | Size | Price |
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NSJ-R20177-100UG | 100ug | £524.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM116
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Fluorescence-activated cell sorting (FACS)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
Storage:
Store the recombinant Human IgG antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Further Information
Application Details:
ELISA: 50ng/well - 200ng/well (Capture); 0.05-0.2ug/ml (Detection),Immunocytochemistry: 0.5-2ug/ml,Immunohistochemistry: 0.5-2ug/ml (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Description:
This recombinant Human IgG antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including G1, G2, G3, and G4. No cross reactivity with other human heavy chains or mouse/rat/goat IgG.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Human IgG was used as the immunogen for this recombinant Human IgG antibody.
Limitation:
This recombinant Human IgG antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01857, P01859, P01860, P01861