Beta Actin Antibody

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NSJ Bioreagents
Product Code: NSJ-R20197
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20197-0.1ML100ul£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM112
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Beta Actin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

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Western blot of human A431 cells using the recombinant Beta Actin antibody at a 1:1000. Predicted molecular weight ~42 kDa.
2 / 3
ICC staining of human HeLa cells using the recombinant Beta Actin antibody at 1:200 (red) and DAPI to stain the nuclei (blue).
3 / 3
IHC staining of FFPE human tonsil with recombinant Beta Actin antibody at 1:1000 (clone RM112). HIER: boil tissue sections in pH 6 citrate buffer or pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Western blot of human A431 cells using the recombinant Beta Actin antibody at a 1:1000. Predicted molecular weight ~42 kDa.
ICC staining of human HeLa cells using the recombinant Beta Actin antibody at 1:200 (red) and DAPI to stain the nuclei (blue).
IHC staining of FFPE human tonsil with recombinant Beta Actin antibody at 1:1000 (clone RM112). HIER: boil tissue sections in pH 6 citrate buffer or pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Western blot: 1:1000,Immunoprecipitation: 1:200,Chromatin IP (ChIP): 1:200,Immunocytochemistry: 1:200,Immunohistochemistry: 1:200 (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Beta Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Beta Actin antibody reacts to b-Actin (ACTB/Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the N-terminus of b-Actin was used as the immunogen for this recombinant Beta Actin antibody.
Limitation:
This recombinant Beta Actin antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P60709