Phosphotyrosine Antibody

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NSJ Bioreagents
Product Code: NSJ-R20195
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20195-100UG100ug£558.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RM111
Regulatory Status: RUO
Target Species: Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Store the recombinant Phosphotyrosine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Images

1 / 4
ICC test of serum-starved human A431 cells non-treated or treated with EGF, using the recombinant Phosphotyrosine antibody at 1:500 (red) and DAPI (blue).
2 / 4
Western blot of serum-starved human A431 cells non-treated or treated with EGF using the recombinant Phosphotyrosine antibody at 1:5000. The blot was exposed to the film at different time points.
3 / 4
IP of EGF-treated human A431 cells by the recombinant Phosphotyrosine antibody at 1:1000 and blotted with an anti-EGFR mAb. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM111 mAb.
4 / 4
Luminex analysis of the recombinant Phosphotyrosine antibody reaction against phosphorylated or non-phosphorylated Serine, Threonine, and Tyrosine. The RM111 mAb reacts only to phosphorylated Tyrosine.
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ICC test of serum-starved human A431 cells non-treated or treated with EGF, using the recombinant Phosphotyrosine antibody at 1:500 (red) and DAPI (blue).
Western blot of serum-starved human A431 cells non-treated or treated with EGF using the recombinant Phosphotyrosine antibody at 1:5000. The blot was exposed to the film at different time points.
IP of EGF-treated human A431 cells by the recombinant Phosphotyrosine antibody at 1:1000 and blotted with an anti-EGFR mAb. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM111 mAb.
Luminex analysis of the recombinant Phosphotyrosine antibody reaction against phosphorylated or non-phosphorylated Serine, Threonine, and Tyrosine. The RM111 mAb reacts only to phosphorylated Tyrosine.

Documents

Further Information

Application Details:
Western blot: 1:1000 -1:5000,Immunoprecipitation: 1:200-1:1000,Chromatin IP (ChIP): 1:200-1:1000,Immunocytochemistry: 1:200-1:500,Immunohistochemistry: 1:200-1:500 (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Phosphotyrosine-BSA conjugate was used as the immunogen for this recombinant Phosphotyrosine antibody.
Limitation:
This recombinant Phosphotyrosine antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant